High-Throughput Real-Time Quantitative PCR System
Exicycler™ 96 is a quantitative real-time PCR instrument that uses our patented technologies, Light Tunnel, an imaging technology based on light excitation, and a 2-D sensor, a fluorescence detection technique using polarization of light, to enhance sensitivity and minimize well-to-well variations.
Application
Gene Expression Analysis / MicroRNA Studies / Copy Number Variation Analysis
Pathogen Detection / Mutational Analysis/ Quantitative & Qualitative GMO Analysis
- Simultaneous analysis of 96 samples
- Excellent uniformity and accuracy of temperature with ±0.3℃ deviation
- Detect even a small amount of DNA with a built-in Arc lamp
- 5-multiplex qPCR without the use of reference dyes
- Shortened experiment time with a faster ramp rate, being a maximum of 5℃/sec (Exicycler™ Fast only)
- Wide dynamic range of more than 9 log
Increased sensitivity & accuracy using our patented reflected light blocking technology
We have applied the fluorescence filtration technology (Korean Patent Number 10-1089045, US patent number 842764) using polarization of light. By applying our patented polarizing beam filter, we were able to eliminate the reflected light from an optical component, which interferes with the fluorescence generated from the samples. Compared with the conventional instruments, our Exicycler™ 96 has greatly increased its sensitivity and accuracy.
Exicycler™ 96 V4 Real-Time Quantitative Thermal Block
Experimental Data
Figure 1. Excellent Uniformity
Fluorescence data using 106
copies of IRF3 gene (FAM
labeled) in each of 96 well
positions. The average Ct of 96
well is 21.8 and the Ct variation
range is 0.19
Figure 2. Wide dynamic range
Graph shows standard curve of
tenfold serial dilutions of 10
copies to 1011 copies MMP9
gene (FAM labeled). The PCR
efficiency generated by the
standard curve is 103%.
Figure 3. Precise discrimination
Fluorescence data from a series
of 1.33-fold dilutions of TMV
gene (106 copies) amplified
using reporter dyes to check
one target: FAM/TMV. The PCR
efficiency generated by the
standard curve is 101%.
Figure 4. Real 5-color Multiplexing
5 target genes can be detected
in a single tube(FAM:T. vaginalis,
TET: M. Hominis, TAMRA: TMV,
Texas Red: HSV type1,
Cyanine5: HSV type2).
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